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browning phenomenon in plant tissue culture and its :

browning phenomenon in plant tissue culture and its

2020-12-20  Plant tissue culture is the maintenance and growth of plant cells, tissues and organs on a suitable culture medium in vitro, e.g., in a test tube or any other suitable vessel. Plant tissue cultures are often classified according to the type of in vitro growth, such as callus and suspension cultures, or the explants used for culture

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(PDF) Browning Treatment in Tissue Culture of ‘Miliang 1 ...

2020-3-17  Browning is a common phenomenon i n the process of tissue culture of various plants, especially woody MEMA 2019 IOP Conf. Series: Materials Science and Engineering 740 (2020) 012194

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Mechanisms and effective control of physiological

Cell and tissue browning is a major impediment of plant culture in vitro. Glycyrrhiza inflata Batalin cells died of lethal browning on Day 9 when cultivated in batch mode.

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The browning in plant tissue culture refers to the release of brown material from the surface to the medium during the initial differentiation or subdivision of the explant, so that the medium gradually turns brown, and the explants also the phenomenon of further browning and death [12]. Browning is ubiquitous in plant tissue culture. This ...

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Mechanisms and effective control of physiological

Browning phenomena are ubiquitous in plant cell cultures that severely hamper scientific research and widespread application of plant cell cultures. Up to now, this problem still has not been well controlled due to the unclear browning mechanisms in plant cell cultures. In this paper, the mechanisms

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Advance of studies on browning and antibrowning

Browning is a phenomenon often observed in tissue cultures of horticultural plants. Studies on browning, phenolic compounds and phenol oxidase, and the influence of factors such as biology, type and composition of the medium and culture conditions are reviewed.

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PAPER OPEN ACCESS

2019-5-18  4. Application of Lipoic Acid in Plant Tissue Culture and Genetic Transformation Browning is a common phenomenon observed in plant tissue culture [22]. It is thought that browning is related to polyphenol oxidase (PPO) in tissues [23]. There are three explanations for enzymatic browning: the regional distribution hypothesis of phenol, phenolase ...

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Studies on stock plant treatment and initiation culture ...

1992-7-1  Recently Read and his co-workers emphasised the importance of stock plant treatment in plant tissue culture (Read et al., 1984, 1986; Read, 1987, 1990); however, its role in controlling oxidative browning in tissue culture of woody species has not been reported.

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Effect of antioxidants on the callus induction and the ...

2017-3-2  The browning of plant tissue and organs is a major constraint in tissue culture of cocoa (Theobroma cacao L.). This study aims to evaluate callogenic and embryogenic potentialities of three genotypes cocoa on culture media supplemented with different types and concentrations of antioxidants.

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An Overview of Arctic Apples: Basic Facts and

2016-10-11  Although this is a natural phenomenon, browning has ... most for its slow browning rate, would show moderate browning in ... selection marker in plant tissue culture. TNOS (1) 0.26 A transcription terminator from A. tumefaciens PCAMV35s 0.65 A transcription promoter from cauliflower

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Research progress on browning phenomenon in tissue

Browning of explants may be easily induced by various factors during tissue culture of medicinal plants, which not only affects the vitality of explants, but also inhibits the callus induction and regeneration, blocking quick accumulation seriously of active components and survival rate of tissue culture. Therefore, it would be important to develop a fast, effective, safe, and economic ...

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How do I reduce the Browning in Tissue culture media

This is the proven method (adding charcoal) in orchid and perennial woody plant tissue culture. Sometimes, activated charcoal also adsorbs some of it and reduce the available concentration.

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EVALUATION OF CALLUS BROWNING AND ... - plant

2015-12-29  Over a culture period of 30 ... This study is important because as plant has various medicinal properties so its secondary metabolites can be collected by invitro callus production at particular time period. ... phenomenon is known to be tissue browning due

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Advance of studies on browning and antibrowning

Browning is a phenomenon often observed in tissue cultures of horticultural plants. Studies on browning, phenolic compounds and phenol oxidase, and the influence of factors such as biology, type and composition of the medium and culture conditions are reviewed.

Read More
Effect of Antioxidants and Carbohydrates in Callus ...

2011-6-9  The tissue browning phenomenon, callus growth, total phenolics content and paclitaxel production, as well as PPO, PO activities in callus of Taxus brevifolia were evaluated in culture media supplemented with ascorbic acid and citric acid (as antioxidants) alsoglucose, fructose and sucrose.

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An Overview of Arctic Apples: Basic Facts and

2016-10-11  Although this is a natural phenomenon, browning has ... most for its slow browning rate, would show moderate browning in ... selection marker in plant tissue culture. TNOS (1) 0.26 A transcription terminator from A. tumefaciens PCAMV35s 0.65 A transcription promoter from cauliflower

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Synergistic Effect of NaCl Pretreatment and PVP on ...

2020-4-22  Browning in plant tissue culture refers to a phenomenon in which the explants release brown substances or phenolics to the medium from its own tissues in the

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Effects of Ascorbic Acid in Controlling Lethal Browning in ...

2021-11-18  Developing tissue culture techniques for B. huillensis will permit the application of biotechnology to its propagation and provide alternative method for its regeneration. The current study was conducted to investigate the effect of antioxidant ascorbic acid in controlling lethal browning caused by oxidized phenols in in vitro culture of ...

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Effect of antioxidants on the callus induction and the ...

2017-3-2  The browning of plant tissue and organs is a major constraint in tissue culture of cocoa (Theobroma cacao L.). This study aims to evaluate callogenic and embryogenic potentialities of three genotypes cocoa on culture media supplemented with different types and concentrations of antioxidants.

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Review on Problems and its Remedy in Plant Tissue Culture

Plant tissue culture is the finest method to propagate in large scale and to protect the rare, endangered and important plant species. It is in urgent to concentrate on conservation of rare, endangered medicinal and commercially important plant species. But the success rate of propagation in this method is attenuate in particular with a few medicinal plants.

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Research progress on browning phenomenon in tissue

Browning of explants may be easily induced by various factors during tissue culture of medicinal plants, which not only affects the vitality of explants, but also inhibits the callus induction and regeneration, blocking quick accumulation seriously of active components and survival rate of tissue culture. Therefore, it would be important to develop a fast, effective, safe, and economic ...

Read More
Advance of studies on browning and antibrowning

Browning is a phenomenon often observed in tissue cultures of horticultural plants. Studies on browning, phenolic compounds and phenol oxidase, and the influence of factors such as biology, type and composition of the medium and culture conditions are reviewed.

Read More
An Overview of Arctic Apples: Basic Facts and

2016-10-11  Although this is a natural phenomenon, browning has ... most for its slow browning rate, would show moderate browning in ... selection marker in plant tissue culture. TNOS (1) 0.26 A transcription terminator from A. tumefaciens PCAMV35s 0.65 A transcription promoter from cauliflower

Read More
Effects of Ascorbic Acid in Controlling Lethal Browning in ...

2021-11-18  Developing tissue culture techniques for B. huillensis will permit the application of biotechnology to its propagation and provide alternative method for its regeneration. The current study was conducted to investigate the effect of antioxidant ascorbic acid in controlling lethal browning caused by oxidized phenols in in vitro culture of ...

Read More
EVALUATION OF CALLUS BROWNING AND ... - plant

2015-12-29  Over a culture period of 30 ... This study is important because as plant has various medicinal properties so its secondary metabolites can be collected by invitro callus production at particular time period. ... phenomenon is known to be tissue browning due

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The role of JrPPOs in the browning of ... - BMC Plant Biology

2021-1-6  Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood. This study investigated explants of ‘Zanmei’ walnut shoot apices cultured in agar (A) or ...

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Effects of Camellia sinensis L. extract and cysteine on ...

cells, however little is known about its impacts in plant cells/tissue culture. In this investigation, we evaluated the biological effects of C. sinensis extract and cysteine on production of paclitaxel using tissue cultures of Taxus brevifolia L. Various calli and its subcultures were cultivated using B5 media in

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Synergistic Effect of NaCl Pretreatment and PVP on ...

2020-4-22  Browning in plant tissue culture refers to a phenomenon in which the explants release brown substances or phenolics to the medium from its own tissues in the

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High Polyphenol Oxidase Activity and Low Titratable ...

Browning of excised plant tissues as well as nutrient media occurs frequently and remains a major basis for recalcitrance in vitro. The severity of browning has varied according to species, tissue or organ, developmental phase of plant, age of tissue or organ, and nutrient medium and other tissue culture variables. When extreme, the

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Phenolic Compositions of Litchi Shoot Tips and Zygotic ...

2016-9-26  lyphenols which cause browning and control of it for the in-vitro culture of explants. During the establishment stage of in-vitro culture, oxidative browning of explants is one of the main drawbacks. Browning is mainly due to the presence of phenolics, re-leased from the cut surfaces of the explants Hu Wang, 1983Lux(-; Endrich et al., 2000).

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